Specificity: Reacts specifically with Sialyl Lewis a containing glycolipids, without detectable reactivity with Lewisª, Lewisb or structurally related substances
as determined by solid-phase binding assays against glycolipid antigens. Shows minimal cross-reactivity (<1%) with Sialyl Lacto-N-tetraosylceramide.
Host Animal: Mouse. Hybridization of NS-1 myeloma
Isotype: IgG1 cells with spleen cells from Balb/c mice.
Source: mouse ascites
Immunogen: Human colorectal adenocarcinoma cell-line SW1116
Format: Purified, Liquid
Purification: Protein A chromatography
Buffer: 0.01M PBS, 0.15M NaCl, pH 7.4
Applications: Capture antibody in serological assays for the research of CA19-9. Immunohistochemistry on frozen or paraffin sections for the detection of
Sialyl Lewisa. Immunochemical detection of Sialyl Lewisa determinant in mucins and glycolipids. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Recommended antibody pair for immunoassay:
Detection: L1C00901, L1C00902
Storage&Shelf life: Store at -20°C for 3 years. Avoid multiple freeze/thaw cycles.
FOR RESEARCH USE ONLY.