Anti-CA15-3 McAb (coating)

Anti-CA15-3 McAb (coating) Specifications

Specificity: recognizes a sialylated carbohydrate epitope of the MUC-1 glycoprotein. Is sensitive to periodate and sialidase treatment, but it does not react with glycolipid extract from the ZR 75-1 cell line. The MUC-1 mucin (Cancer Antigen 15-3) is secreted from tumor cells. The apo-protein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism).

Clone: CA008

Host Animal: Mouse. Hybridization of P3X63-Ag8.653

Isotype: IgG1, myeloma cells with spleen cells from Balb/c mice.

Source: Mouse Ascites

Immunogen: Human breast cancer cell line ZR 75-1

Format: Purified, Liquid

Purification: Ion exchange

Concentration&Lot: 5.5 mg/ml

Lot: L180401

Buffer: 150mM NaCl 10mM Tris PH7.4

Preservative: 0.05%NaN3

Applications: Capture antibody in serological assays for the research of antigen MUC-1. Forms a suitable pair with CA009, Catalog # L1C00801as detection. Immunohistochemistry on frozen or paraffin sections for the detection of MUC-1. Immunochemical detection of MUC-1 protein. Each laboratory should determine an optimum working titer for use in its particular application. 

Storage&Shelf life: Store at 2~8 C for 3 years. Avoid multiple freeze/thaw cycles.

FOR RESEARCH USE ONLY