Rapid Diagnostic Test for Dengue

Dengue Test Kit Procedure

For qualitative rapid detection of dengue virus IgM and IgG antibodies in human serum, plasma or whole blood. Can be used in clinical laboratories to test results within 15 minutes in patients with persistent fever and dengue symptoms.

Dengue Fever Test Kit Results

1. It is used for qualitative rapid detection of dengue virus IgM and IgG antibodies in human serum, plasma or whole blood. Results can be detected within 15 minutes.

2. It is used to qualitatively detect the IgM antibody of dengue virus in the serum of the population, and it is used for the auxiliary diagnosis of patients with dengue fever symptoms with persistent fever in clinical laboratories.

3. For qualitative detection of IgG antibodies against dengue virus (serotypes 1, 2, 3 and 4) in serum. It is used for auxiliary diagnosis of secondary dengue infection in clinical laboratory.

4. For qualitative detection of dengue virus NS1 antigen (serotypes 1, 2, 3 and 4) in serum. It is used for the auxiliary diagnosis of dengue fever patients with persistent fever in clinical laboratory.

5. For qualitative detection of IgG antibodies against dengue virus (serotypes 1, 2, 3 and 4) in serum, for clinical laboratory auxiliary diagnosis of patients with persistent fever and dengue infection symptoms or contact history.

6. For qualitative detection of dengue virus IgM and IgG antibodies in serum. A primary infection can be distinguished from a secondary infection.

A Dengue NS1 test  kit positive result confirms dengue virus infection without providing serotype information.

A Dengue NS1 test  kit  negative result does not rule out infection. People with negative NS1 results should be tested for the presence of dengue IgM antibodies to determine possible recent dengue exposure.

Application Of Dengue Fever Test Kit

1. For the detection of dengue virus secondary infection.

2. For the diagnosis of active dengue virus infection.

3. For the diagnosis of dengue virus endemic areas.

4. It can be used 2-5 days after the onset of the disease.

Precautions Of Dengue Fever Test Kit

1. For in vitro diagnostic use and professional use only.

2. Read the package insert instruction before use the kit.

3. Do not use beyond the expiration date which appears on the package label.

4. Do not open the sealed pouch, unless ready to conduct the assay

5. Bring all reagents to room temperature (15°C-30°C) before use.

6. Haemolized blood may be used for the testing, but do not take precipitants.

7. Wear protective clothing and disposable gloves while assaying samples. Wash hands thoroughly after performing the test.

8. Handle all specimens as if they contain infectious agents. When the assay procedure is completed, dispose of specimens carefully after autoclaving them for at least one hour. Alternatively, they can be treated with 0.5 to 1% solution of sodium hypochlorite for one hour before disposal.

9. Do not smoke, drink, or eat in areas where specimens or kit reagents are being handled.

10. Read result after 30 minutes may give erroneous results.

11. Do not perform the test in a room with strong air flow, an electric fan or strong air-conditioning.

12. As with all diagnostic tests, a definitive clinical diagnosis should not be based on the results of a single test, but should only be made by the physician, after all, clinical and laboratory findings have been evaluated.

13. Excess sample volume (>5µL) can give false positives.

Dengue virus is an enveloped, single-stranded, positive-sense RNA virus that comprises four related but distinct serotypes (Den 1, 2, 3, and 4). The virus is transmitted by mosquitoes of the daytime-biting Stegomyia family, principally Aedes aegypti and Aedes albopictus. Today, more than 2.5 billion people living in the areas of tropical Asia, Africa, Australia and the Americas are at risk for dengue infection. 

An estimated 100 million cases of dengue fever and 250,000 cases of life-threatening dengue hemorrhagic fever occur annually on a worldwide basis. Serological detection is a common method for the diagnosis of infection with the dengue virus. Anti-dengue virus IgM begins to appear at 3 days after initial exposure and circulates for about 30-60 days. Anti-dengue virus IgG is elevated around 7 days, peaks at 2-3 weeks, and persists for life. Detection of antigens released during virus replication in the infected patient shows very promising results; it enables diagnosis from the first day after the onset of fever up to day 9 once the clinical phase of the disease is over, thus allowing early detection and prompt treatment. For information on dengue rapid test kit prices, please contact us.