Good's buffer has the following characteristics: 1. High water solubility; 2. Low cell membrane permeability; 3. stable acid-base dissociation constant; 4. Low metal chelating capacity; 5. High chemical stability; 6. Low absorption spectra in ultraviolet and visible region;7. Low salt effect; 8. Easy to make high purity salt. They are essential raw materials for in vitro diagnostic reagents in the process of production.
Tris aminomethane is an organic compound with the chemical formula C4H11NO3 and white crystalline particles. Can be used as biological buffer; used to prepare buffers for gel electrophoresis. As an alkaline drug, it is used for the correction of acidosis without causing an increase in carbon dioxide retention.
Tris was used for protein crystal growth at different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffers. In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators and some pharmaceuticals. Tris is also used as a titration standard.
Tris, also known as tris(hydroxymethyl)aminomethane or THAM, is an organic compound with the molecular formula C4H11NO3. It is a white, crystalline solid that is highly soluble in water. Tris is classified as an amine and is often referred to as a tertiary amine due to the presence of three hydroxyl groups (-OH) attached to the nitrogen atom. Tris hydroxymethyl aminomethane has a high boiling point and low vapor pressure, allowing it to remain stable at elevated temperatures. It is also a weak base, readily accepting protons to act as a pH buffer in aqueous solutions. Tris has a pKa value of approximately 8.1, meaning it can maintain a stable pH in the range of 7.0 to 9.2.
Tris, short for tris(hydroxymethyl)aminomethane, has diverse applications in various fields such as biochemical, microbiological, pharmaceutical, and molecular biology. Its primary use is as a buffer substance or a component in buffer solutions. It is utilized in the formulation of injection and infusion solutions, vaccines, eye drops, creams, and gels to improve their stability as an excipient. Tris (hydroxymethyl)aminomethane acts as a pH buffer, regulating and counteracting small fluctuations in the pH of pharmacological solutions. This feature is crucial in drugs prepared in liquid form, as pH affects solubility, activity, stability, and biological tolerability of the formulation. Notably, Tris (tromethanol) is employed as a component in some COVID-19 vaccines.