Recombinant TB-16, TB-38 & ESAT-6 Fusion Antigen
- Analyte
- 16 kDa · 38 kDa · ESAT-6 combined antigen
- Source
- Recombinant (M. tuberculosis)
- Reference
- P9WMK1 · P9WGU1 · P9WNK7
- Documentation
- Lot-specific CoA with every batch · SDS/MSDS and HPLC/MS data on request
- Availability
- Evaluation quantity, standard and bulk — quoted per order
A triple fusion of the 16 kDa antigen, the 38 kDa antigen and ESAT-6. Combining several TB antigens in one polypeptide is a common way to broaden coverage in immunogen preparation and antibody screening, while keeping a single reagent to track and document. As with all our antigens, the manufacturer's specification sheet is published in full and the lot CoA travels with every batch.
| Category | Recombinant antigen / IVD raw material |
|---|---|
| Species / origin | Mycobacterium tuberculosis, recombinant (E. coli expressed) |
| Aliases | 16 kDa · 38 kDa · ESAT-6 combined antigen |
| Molecular weight | Triple fusion; ESAT-6 component ~6 kDa |
| Application | Immunogen preparation, antibody panel screening, TB assay development |
| Format | Purified protein solution in 20 mM Tris-HCl, pH 8.0; untagged |
| Purity | >90% (manufacturer's published specification; the lot CoA governs) |
| Endotoxin | Not stated on the manufacturer's specification; ask us if your application requires a figure |
| Form | Liquid, frozen; ships frozen |
| Storage | -20 °C, 3-year shelf life; avoid repeated freeze/thaw |
| Handling note | The manufacturer states that all cultures have tested negative for HBsAg, HCV, HIV-1 and HIV-2, and that no test guarantees a product to be non-infectious — all material should be handled as potentially infectious |
| Packaging | Evaluation size / 1 mg / custom — bulk quantities on request |
| Documentation | Lot-specific CoA; the manufacturer's specification sheet is published on this page |
| Price | On request |
Representative specification (manufacturer's published data)
Representative specification from the manufacturer's published specification sheet. The lot shipped to you is described by its own Certificate of Analysis. Shown so you can judge fit before you enquire — these are the manufacturer's published figures for the catalogue items we source, not a substitute for the Certificate of Analysis of the lot you receive. The full set for every item we publish is on published specifications.
Recombinant TB-16, TB-38 & ESAT-6 antigen
Manufacturer catalogue L1H01303 · example lot shown: L170901
| Product | Recombinant TB-16, TB-38 and ESAT-6 antigen |
|---|---|
| Grade | Antigen Grade |
| Source / expression | E. coli |
| Format | Purified, protein solution in Tris-HCl |
| Purity | >90% |
| Concentration | 2.9 mg/ml |
| Buffer | 20 mM Tris-HCl, pH 8.0 ± 0.02 |
| Preservative | None |
| Stated applications | EIA, CLIA, IP, IHC, WB |
| Storage & shelf life | -20 °C for 3 years; avoid multiple freeze/thaw cycles |
| Culture screening | The manufacturer states that all cultures have tested negative for HBsAg, HCV, HIV-1 and HIV-2, and that no test guarantees a product to be non-infectious — all material should be considered potentially infectious |
| Manufacturer's use statement | For research only |
Triple fusion covering all three antigens — a common immunogen format when an antibody panel against several TB targets is being raised from one reagent. Note the culture-screening statement: it is the manufacturer's own wording, reproduced rather than paraphrased.
Reference sequence & construct notes
| Reference (UniProt) | P9WMK1 · P9WGU1 · P9WNK7 |
|---|---|
| Commonly used construct | Triple fusion: HspX (Rv2031c), PstS1 (Rv0934) and ESAT-6 (esxA, Rv3875) |
| Molecular weight | Fusion construct |
| Tag options | Untagged / carrier-free, His, hFc — confirmed per product |
| Purity methods | SDS-PAGE and HPLC / SEC-HPLC, reported per lot |
| Endotoxin grades | < 1.0 EU/µg standard; < 0.1 EU/µg for cell-based work |
| Form & storage | Lyophilised; -20 °C to -80 °C; see the CoA for the lot |
Reference sequence information is given to help you choose a construct, not as a specification of the material shipped. Construct, tag, expression system and lot data are stated on the batch CoA — tell us if your assay is sensitive to any of these and we will confirm before you order.
Related materials in this family
Recombinant TB CFP-10 Antigen →Recombinant TB ESAT-6 Antigen →Recombinant TB-16 & TB-38 Fusion Antigen →Ordering options for this item
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Request an evaluation sample Request a quotation Request documents
One subject line each — send it as it opens and we will have what we need to answer. If you already hold a lot number, add it and we will locate its record.
Questions buyers ask about this item
Can this be supplied in a different format, grade or pack size?
The format, grade and pack size actually available are confirmed per item. If your assay depends on a specific tag, on carrier-free material, or on particular impurity limits, state it in your enquiry and we will confirm what is available before you commit — rather than after. For a buffer or preservative line, tag and expression-system fields do not apply.
What documentation accompanies this product?
A lot-specific Certificate of Analysis with every batch, and a commercial invoice and packing list with every shipment. HPLC / MS data, SDS/MSDS and a certificate of origin are available on request. See the documentation centre.
What are the lead time and minimum quantity?
Lead time and minimum order quantity depend on availability, grade and destination, and are stated on your quotation. Evaluation quantities are available for research groups qualifying a lot against an existing assay.
Can you reserve the same lot for my reorders?
Where stock allows, yes — a consistent lot avoids re-validation work. Ask us at the time of your first order and we will confirm availability rather than promising it later.
For research use and in-vitro diagnostic development only. Not for human or animal therapeutic use. Specifications are stated per lot on the accompanying CoA. Please note the boundary precisely: our sourcing serves research and assay development, and the manufacturer's own specification sheets state “for research use only, not for use in diagnostic procedures”. If your programme needs material released under a particular quality system or with a specific regulatory status, tell us before you order — we will confirm what the manufacturer can actually provide rather than assume it.
Not sure which grade to specify? Read the specification guide — purity, endotoxin, tag format and expression system all change what the material can be used for.
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