Recombinant Cardiac Troponin I (cTnI)
- Analyte
- TNNI3 · cardiac troponin I
- Source
- Recombinant protein
- Reference
- P19429
- Documentation
- Lot-specific CoA with every batch · SDS/MSDS and HPLC/MS data on request
- Availability
- Evaluation quantity, standard and bulk — quoted per order
Cardiac troponin I is the standard marker of myocardial injury. Recombinant cTnI with consistent lot-to-lot performance is used as assay antigen, calibrator and control material in high-sensitivity troponin immunoassays and in rapid tests.
| Category | Recombinant protein / IVD biomarker raw material |
|---|---|
| Species / origin | Human recombinant |
| Aliases | TNNI3 · cardiac troponin I |
| Molecular weight | ~24 kDa |
| Application | hs-cTnI assay development, calibrators & controls |
| Format | Carrier-free and tagged formats available — confirmed per product |
| Purity | Reported per lot on the CoA. Methods used: SDS-PAGE (subunit purity by size) and HPLC / SEC-HPLC (monomer and aggregate distribution) — ask us which method a figure came from |
| Endotoxin | Not a fixed catalogue value. Grades available on request: < 1.0 EU/µg (standard) or < 0.1 EU/µg (cell-based work); the figure for the lot shipped is stated on its CoA |
| Form | Lyophilised (liquid formats on request) |
| Storage | -20 °C to -80 °C |
| Packaging | Evaluation size / 1 mg / custom — bulk quantities on request |
| Documentation | CoA with every batch; MS, HPLC and SDS data on request |
| Price | On request |
Representative specification (manufacturer's published data)
Representative specification from the manufacturer's published specification sheet. The lot shipped to you is described by its own Certificate of Analysis. Shown so you can judge fit before you enquire — these are the manufacturer's published figures for the catalogue items we source, not a substitute for the Certificate of Analysis of the lot you receive. The full set for every item we publish is on published specifications.
Recombinant antigen
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Recombinant human cardiac troponin I (cTnI) |
|---|---|
| Molecular weight | Approximately 30 kDa (as stated by the manufacturer) |
| Source / expression system | Expressed in E. coli |
| Purity | >90% (SDS-PAGE) |
| Concentration | 1.1 mg/ml |
| Buffer | 10 mM PBS, pH 7.4, 0.01% SKL |
| Preservative | None |
| Stated applications | Standard in immunoassay; immunogen for antisera production; tracer for iodination |
| Storage & shelf life | -20 °C for 3 years; avoid multiple freeze/thaw cycles |
| Manufacturer's use statement | For research use only. Not for use in diagnostic procedures. |
The E. coli-expressed recombinant antigen is the usual choice for calibrator preparation: traceable to a stated construct and free of the donor-screening obligations attached to human tissue-derived material.
Native antigen (human tissue-derived)
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Purified native human cardiac troponin I |
|---|---|
| Source | Human heart tissue |
| Immunological activity | Confirmed by reaction with a monoclonal antibody specific for the cardiac troponin I isoform in immunoblotting |
| Format | Purified, liquid |
| Concentration | 4.4 mg/ml |
| Buffer | 20 mM Tris, pH 7.5, with 5 mM EDTA and 15 mM mercaptoethanol |
| Preservative | None |
| Stated applications | Standard in immunoassay |
| Storage & shelf life | -20 °C for 3 years; avoid multiple freeze/thaw cycles |
| Donor screening | The tissue donor was tested negative for HIV-1, HIV-2, HCV antibodies, HBsAg and syphilis. No test can give complete assurance that infectious agents are absent, so the material must be handled as potentially infectious. |
| Manufacturer's use statement | For research use only. Not for use in diagnostic procedures. |
Purified from human heart tissue rather than expressed recombinantly, so it presents the native isoform but carries the handling obligations of human-derived material — read the donor-screening statement before you plan the experiment.
Matched antibody pair
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Mouse monoclonal anti-cTnI — recommended capture antibody |
|---|---|
| Clone | NI001 |
| Host / isotype | Mouse, IgG1 (hybridoma of Sp2/0 myeloma with Balb/c spleen cells) |
| Source | Ascites |
| Immunogen | Human cTnI |
| Format | Purified, liquid |
| Purification | Protein A chromatography |
| Concentration | 6 mg/ml |
| Buffer | 10 mM PBS, pH 7.4 |
| Preservative | None |
| Specificity | Reacts with free cardiac troponin I (cTnI) |
| Stated applications | cTnI immunoassay, immunoprecipitation, immunohistochemistry, immunoaffinity purification, Western blot |
| Recommended pair | A published sandwich pairing is available for this clone — ask us for the recommended capture/detection combination for your format |
| Storage & shelf life | -20 °C for 3 years; avoid multiple freeze/thaw cycles |
The manufacturer states a recommended sandwich pairing for this capture clone — ask us and we will confirm the detection partner recommended for your format. A published pairing removes one variable from your optimisation, provided your epitopes and matrix match the conditions it was intended for.
Reference sequence & construct notes
| Reference (UniProt) | P19429 |
|---|---|
| Commonly used construct | TNNI3, cardiac troponin I — full-length and truncated constructs both used |
| Molecular weight | ~24 kDa |
| Tag options | Untagged / carrier-free, His, hFc — confirmed per product |
| Purity methods | SDS-PAGE and HPLC / SEC-HPLC, reported per lot |
| Endotoxin grades | < 1.0 EU/µg standard; < 0.1 EU/µg for cell-based work |
| Form & storage | Lyophilised; -20 °C to -80 °C; see the CoA for the lot |
Reference sequence information is given to help you choose a construct, not as a specification of the material shipped. Construct, tag, expression system and lot data are stated on the batch CoA — tell us if your assay is sensitive to any of these and we will confirm before you order.
Related materials in this family
Human Recombinant Soluble ST2 (sST2 / IL1RL1) →Purified D-dimer Antigen →Ordering options for this item
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Questions buyers ask about this item
Can this be supplied in a different format, grade or pack size?
The format, grade and pack size actually available are confirmed per item. If your assay depends on a specific tag, on carrier-free material, or on particular impurity limits, state it in your enquiry and we will confirm what is available before you commit — rather than after. For a buffer or preservative line, tag and expression-system fields do not apply.
What documentation accompanies this product?
A lot-specific Certificate of Analysis with every batch, and a commercial invoice and packing list with every shipment. HPLC / MS data, SDS/MSDS and a certificate of origin are available on request. See the documentation centre.
What are the lead time and minimum quantity?
Lead time and minimum order quantity depend on availability, grade and destination, and are stated on your quotation. Evaluation quantities are available for research groups qualifying a lot against an existing assay.
Can you reserve the same lot for my reorders?
Where stock allows, yes — a consistent lot avoids re-validation work. Ask us at the time of your first order and we will confirm availability rather than promising it later.
For research use and in-vitro diagnostic development only. Not for human or animal therapeutic use. Specifications are stated per lot on the accompanying CoA. Please note the boundary precisely: our sourcing serves research and assay development, and the manufacturer's own specification sheets state “for research use only, not for use in diagnostic procedures”. If your programme needs material released under a particular quality system or with a specific regulatory status, tell us before you order — we will confirm what the manufacturer can actually provide rather than assume it.
Not sure which grade to specify? Read the specification guide — purity, endotoxin, tag format and expression system all change what the material can be used for.
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