Human Recombinant Soluble ST2 (sST2 / IL1RL1)
- Analyte
- IL-33 receptor · IL1RL1 · soluble ST2
- Source
- Recombinant protein
- Reference
- Q01638
- Documentation
- Lot-specific CoA with every batch · SDS/MSDS and HPLC/MS data on request
- Availability
- Evaluation quantity, standard and bulk — quoted per order
Soluble ST2 (sST2) is a circulating biomarker of cardiac stress and adverse remodelling, providing prognostic information in heart failure independently of BNP and troponin. As the soluble receptor of IL-33 it is also a central reagent for IL-33/ST2 signalling research in immunology and inflammation. Supplied as a high-purity recombinant protein with lot-specific CoA.
| Category | Recombinant protein / IVD biomarker raw material |
|---|---|
| Species / origin | Human recombinant |
| Aliases | IL-33 receptor · IL1RL1 · soluble ST2 |
| Molecular weight | Construct-dependent — stated on the batch CoA |
| Application | Immunoassay development, calibrators & controls, IL-33/ST2 signalling studies |
| Format | Carrier-free and tagged formats available — confirmed per product |
| Purity | Reported per lot on the CoA. Methods used: SDS-PAGE (subunit purity by size) and HPLC / SEC-HPLC (monomer and aggregate distribution) — ask us which method a figure came from |
| Endotoxin | Not a fixed catalogue value. Grades available on request: < 1.0 EU/µg (standard) or < 0.1 EU/µg (cell-based work); the figure for the lot shipped is stated on its CoA |
| Form | Lyophilised (liquid formats on request) |
| Storage | -20 °C to -80 °C |
| Packaging | Evaluation size / 1 mg / custom — bulk quantities on request |
| Documentation | CoA with every batch; MS, HPLC and SDS data on request |
| Price | On request |
Representative specification (manufacturer's published data)
Representative specification from the manufacturer's published specification sheet. The lot shipped to you is described by its own Certificate of Analysis. Shown so you can judge fit before you enquire — these are the manufacturer's published figures for the catalogue items we source, not a substitute for the Certificate of Analysis of the lot you receive. The full set for every item we publish is on published specifications.
Antigen — recombinant human sST2
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Recombinant human ST2 antigen |
|---|---|
| Molecular weight | 40 kDa (as stated by the manufacturer) |
| Source / expression system | E. coli |
| Purification | Affinity purified |
| Concentration | 1.4 mg/ml |
| Buffer | 10 mM PBS, pH 7.4, 1 mM EDTA, 0.1% SKL, 0.2% NaN₃ |
| Preservative | 0.2% NaN₃ |
| Stated applications | Standard in immunoassay; immunogen for antisera production; tracer for iodination |
| Storage & shelf life | -20 °C for 3 years; avoid multiple freeze/thaw cycles |
| Manufacturer's use statement | For research use only. Not for use in diagnostic procedures. |
Recombinant sST2 is the calibrator antigen. The manufacturer's stated applications include use as an immunoassay standard, as an immunogen for antisera production, and as a tracer for iodination — the last two matter if you are raising new antibodies rather than buying existing ones.
Matched antibody — suggested capture
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Mouse monoclonal anti-sST2 (suggested capture) |
|---|---|
| Host / isotype | Mouse, IgG |
| Source | Ascites |
| Immunogen | Purified human sST2 antigen |
| Format | Affinity purified, liquid, Protein A chromatography |
| Purity | >90% (SDS-PAGE) |
| Concentration | 4.38 mg/ml (OD280 nm) |
| Buffer | 0.01 M PB, 150 mM NaCl, pH 7.4 |
| Preservative | None |
| Stated applications | ELISA and Western blot; working titre to be determined by the user |
| Storage | -20 °C |
Coating antibody from the same manufacturer and raised against the same antigen as the material above, which is the practical condition for a sandwich pair to behave consistently.
Matched antibody — labelling / detection
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Mouse monoclonal anti-sST2 (labelling / detection) |
|---|---|
| Host / isotype | Mouse, IgG |
| Source | Ascites |
| Immunogen | Purified human sST2 antigen |
| Format | Affinity purified, liquid, Protein A chromatography |
| Purity | >90% (SDS-PAGE) |
| Concentration | 5.8 mg/ml (OD280 nm) |
| Buffer | 0.01 M PB, 150 mM NaCl, pH 7.4 |
| Preservative | None |
| Stated applications | ELISA and Western blot; working titre to be determined by the user |
| Storage | -20 °C |
Supplied unlabelled and unconjugated, so the labelling chemistry and label-to-protein ratio remain yours to control — which matters when the label affects the epitope you are reading.
Polyclonal alternative
Published specification for this item. The lot shipped to you is described by its own Certificate of Analysis, which travels with every shipment.
| Product | Rabbit polyclonal anti-sST2 |
|---|---|
| Host / isotype | Rabbit, IgG |
| Source | Ascites |
| Immunogen | Purified human sST2 antigen |
| Format | Affinity purified, liquid, Protein A chromatography |
| Purity | >90% (SDS-PAGE) |
| Concentration | 9 mg/ml (OD280 nm) |
| Buffer | 0.01 M PB, 150 mM NaCl, pH 7.4 |
| Preservative | None |
| Stated applications | ELISA and Western blot; working titre to be determined by the user |
| Storage | -20 °C |
A rabbit polyclonal raised against the same antigen. Useful as a capture in formats where a broad epitope repertoire is an advantage, or as a secondary reagent in detection.
Reference sequence & construct notes
| Reference (UniProt) | Q01638 |
|---|---|
| Commonly used construct | IL1RL1 (ST2), isoform 2 — commonly expressed as Lys19–Phe328 |
| Molecular weight | Predicted and apparent molecular weight depend on the construct actually shipped; both are stated on the batch CoA |
| Tag options | Untagged / carrier-free, His, hFc — confirmed per product |
| Purity methods | SDS-PAGE and HPLC / SEC-HPLC, reported per lot |
| Endotoxin grades | < 1.0 EU/µg standard; < 0.1 EU/µg for cell-based work |
| Form & storage | Lyophilised; -20 °C to -80 °C; see the CoA for the lot |
Reference sequence information is given to help you choose a construct, not as a specification of the material shipped. Construct, tag, expression system and lot data are stated on the batch CoA — tell us if your assay is sensitive to any of these and we will confirm before you order.
Ordering options for this item
Three different requests, three different answers — pick the one that matches where you are.
Request an evaluation sample Request a quotation Request documents
One subject line each — send it as it opens and we will have what we need to answer. If you already hold a lot number, add it and we will locate its record.
Questions buyers ask about this item
Can this be supplied in a different format, grade or pack size?
The format, grade and pack size actually available are confirmed per item. If your assay depends on a specific tag, on carrier-free material, or on particular impurity limits, state it in your enquiry and we will confirm what is available before you commit — rather than after. For a buffer or preservative line, tag and expression-system fields do not apply.
What documentation accompanies this product?
A lot-specific Certificate of Analysis with every batch, and a commercial invoice and packing list with every shipment. HPLC / MS data, SDS/MSDS and a certificate of origin are available on request. See the documentation centre.
What are the lead time and minimum quantity?
Lead time and minimum order quantity depend on availability, grade and destination, and are stated on your quotation. Evaluation quantities are available for research groups qualifying a lot against an existing assay.
Can you reserve the same lot for my reorders?
Where stock allows, yes — a consistent lot avoids re-validation work. Ask us at the time of your first order and we will confirm availability rather than promising it later.
For research use and in-vitro diagnostic development only. Not for human or animal therapeutic use. Specifications are stated per lot on the accompanying CoA. Please note the boundary precisely: our sourcing serves research and assay development, and the manufacturer's own specification sheets state “for research use only, not for use in diagnostic procedures”. If your programme needs material released under a particular quality system or with a specific regulatory status, tell us before you order — we will confirm what the manufacturer can actually provide rather than assume it.
Not sure which grade to specify? Read the specification guide — purity, endotoxin, tag format and expression system all change what the material can be used for.
Request a Quote or Sample
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