Tris HCl is a very important biological buffer, which is widely used in biological system diagnosis industry, such as electrophoresis and protein biotechnology. Tris HCl has a pKa of 8.1, which makes it very suitable for biologics formulation.
Trimethylolmethane hydrochloride buffer can be used in a variety of biological applications, including medium, electrophoresis separation and diagnostic reagent formulation. It can provide a stable weak alkaline environment (7.0-9.1), and the pH value of all buffers depends on temperature and concentration. For Tris HCl buffer, the pH increases by about 0.03 units for every 1℃ increased in temperature and decreases by 0.03-0.05 units for dilute every 10 times.
Packaging Size: 500g, 1.0Kg, 5.0Kg, 12.5Kg, 25Kg
|Appearance (Color)||White Crystalline Powder|
|APHA (Color, 1.0M aqueous solution)||≤20|
|Solubility (Turbidity, 5% aqueous solution)||Clear|
|Assay (Titration by NaOH)||≥99.0%|
|Infrared Spectrum Complies||Conforms to Structure|
|pH 1% H2O||4.2-5.0 (25℃)|
|UV Absorbance 260mm 10% w/w||absorbance≤0.03|
|UV Absorbance 280mm 10% w/w||absorbance≤0.03|
|UV Absorbance 260mm 10% w/w||absorbance≤0.05|
|UV Absorbance 290mm 10% w/w||absorbance≤0.05|
|Loss on drying (105℃ for 3 Hours) WT%||≤0.5%|
|Sulfated Ash (650℃ for 3 Hours) Wt%||≤0.1%|
|Heavy Metals Pb||≤5ppm|
Biologic Reagent (BR) and Pharma grade can be available as requested, with DNase-Endonuclease/Exonuclease test and DNase –Nickase, Bioburden <=100 CFU/g, Yeast & Mold <=100 CFU/g, Endotoxin <=50EU/g.
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