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Tris HCl is a very important biological buffer, which is widely used in biological system diagnosis industry, such as electrophoresis and protein biotechnology. Tris HCl has a pKa of 8.1, which makes it very suitable for biologics formulation.
Trimethylolmethane hydrochloride buffer can be used in a variety of biological applications, including medium, electrophoresis separation and diagnostic reagent formulation. It can provide a stable weak alkaline environment (7.0-9.1), and the pH value of all buffers depends on temperature and concentration. For Tris HCl buffer, the pH increases by about 0.03 units for every 1℃ increased in temperature and decreases by 0.03-0.05 units for dilute every 10 times.
Formula: C4H11NO3.HCl
M.W.: 157.6
Packaging Size: 500g, 1.0Kg, 5.0Kg, 12.5Kg, 25Kg
| Test | Specification |
| Appearance (Color) | White Crystalline Powder |
| APHA (Color, 1.0M aqueous solution) | ≤20 |
| Solubility (Turbidity, 5% aqueous solution) | Clear |
| Melting point | 150-155°C |
| Assay (Titration by NaOH) | ≥99.0% |
| Infrared Spectrum Complies | Conforms to Structure |
| pH 1% H2O | 4.2-5.0 (25℃) |
| UV Absorbance 260mm 10% w/w | absorbance≤0.03 |
| UV Absorbance 280mm 10% w/w | absorbance≤0.03 |
| UV Absorbance 260mm 10% w/w | absorbance≤0.05 |
| UV Absorbance 290mm 10% w/w | absorbance≤0.05 |
| Loss on drying (105℃ for 3 Hours) WT% | ≤0.5% |
| Sulfated Ash (650℃ for 3 Hours) Wt% | ≤0.1% |
| Heavy Metals Pb | ≤5ppm |
| Iron, Fe | ≤5ppm |
Biologic Reagent (BR) and Pharma grade can be available as requested, with DNase-Endonuclease/Exonuclease test and DNase –Nickase, Bioburden <=100 CFU/g, Yeast & Mold <=100 CFU/g, Endotoxin <=50EU/g.
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2023/05/26
