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Use of Malaria Test Kits

Malaria is an infectious disease caused by the parasites of plasmodium parasites in the human body. Infected by malaria mosquito bites or transfusion of the blood of a person carrying malaria parasites. Different malaria parasites cause vivax, vivax, falciparum and ovarian malaria respectively. Malaria is mainly manifested as periodic regular attacks, body chills, fever, and sweating. After multiple attacks for a long period of time, it can cause pelvic blood and splenomegaly. The incidence of children is high, mostly in summer and autumn. Malaria is endemic in 102 countries and regions, especially in some countries in Africa, Southeast Asia, and Central and South America. The mortality rate of falciparum malaria is extremely high. Therefore, it is very important to exclude it through detection at the first time.


1. Inspection principle of malaria test kit


This reagent uses immunochromatographic analysis technology and double antibody sandwich method to detect specific soluble proteins PF lactate dehydrogenase and pan lactate dehydrogenase contained in red blood cells or released outside of red blood cells.


When using the malaria test kit to detect, add 5 microliters of whole blood sample to the reagent loading hole, and then add 4 drops of lysate. The lysed sample is chromatographed upward under the capillary effect. If the specimen contains PFIPAN lactate dehydrogenase, it will react with the pre-labeled lactate dehydrogenase antibody to form a complex, which will be captured by the PF/PAN lactate dehydrogenase antibody pre-immobilized on the membrane under chromatographic action. 1 or 2 red reaction lines are formed in the area, which is a positive result at this time; if the specimen does not contain PFIPAN lactate dehydrogenase, there is no red reaction line in the detection area, and it is a negative result at this time. Regardless of whether the substance to be tested is present in the sample, a red reaction line will be formed in the quality control area. This is a standard for judging whether the amount of sample is sufficient and the chromatography process is normal. It is also an internal control standard for reagents.


2. Detection method of malaria test kit


Please read the manual of the malaria test kit before the test. The test should be performed at room temperature.


(1) Tear along the cut of the aluminum film bag, take out the reagent card, lay it flat, and make a mark.


(2) Take 5 microliters of whole blood sample and drop it vertically into well A, and add 4 drops of lysate into well B at the same time.


(3) Observe and display the results within 15 minutes; the results displayed after 30 minutes have no clinical significance.


3. Interpretation of the results of the malaria test kit


positive:


(1) Two red reaction lines, that is, one red reaction line appears in the detection area (T1) and the control area (C), indicating that Plasmodium falciparum is infected.


(2) Three red reaction lines, that is, one red reaction line appears in the detection area (T1, T2) and the control area (C). It indicates infection with Plasmodium falciparum, but joint infection with other types of Plasmodium is not excluded.


(3) Two red reaction lines, that is, one red reaction line appears in the detection area (T2) and the control area (C) of the malaria test kit, indicating that Plasmodium infection other than falciparum.


negative:


A red reaction line only appears in the control area (C).